HELP	ARTD-Arts   BSSD-Behav. & Social Sci.   HUMD-Humanities    NSLD-Nat. Sci. with Lab   NSND-Nat. Sci. NO lab   [M] - Global & Cultural Prospective CE -- Credit by Exam available     (...only)  -- offered only on indicated campus(es)     G - Germantown    R - Rockville    TP - Takoma Park/Silver Spring
Course	Course Title	Semester Hours	Subject
BT 101	INTRODUCTION TO BIOTECHNOLOGY	2 semester hours	BT-Biotechnology
Course Description:    Designed to introduce the student to the concepts of biotechnology as they relate to working in the biotechnology industry. Included are overviews of product development, GLP and cGMP, employer expectations, basic laboratory math and statistics, buffer preparation, handling of equipment and reagents (e.g., enzymes), introduction to experimental design, safety considerations, ethics at the workplace, and introduction to relevant biotech databases available on the Web. Two hours each week.
BT 115	INSTRMTION FOR THE BIOTECH LAB	3 semester hours	BT-Biotechnology
Course Description:    A survey of the theory and practice of laboratory instrumentation with emphasis on biotechnology applications. Principles of measurement and calibration using electronic balances, volumetric apparatus, and pH meters will be presented in the context of a GLP/cGMP environment. Quantitative analysis using UV-Vis spectrophotometry and fluorometric measurements will be described. Separation of biomolecules by chromatographic and electrophoretic methods will be presented. Laboratory robotics will be briefly introduced. (G only) PREREQUISITE: CH 101 or consent of department. Two hours lecture, three hours laboratory each week.
BT 115L	INSTRMTION FOR THE BIOTECH LAB	No Credit	BT-Biotechnology
Course Description:    Laboratory component of BT 115.
BT 117	CELL CULTURE & CELL FUNCTION	3 semester hours	BT-Biotechnology
Course Description:    An introduction to fundamental methods used to grow animal cells in culture and associated principles of cell structure and function. Topics in this course include aseptic technique, preparation and use of various culture media, cell counting and dilution, maintenance and propagation of cell lines, origin and uses of various cell lines, contamination, cell staining techniques, and quality control. A survey of metabolism, cell structure and function, growth factors and signal transduction. (G only) PREREQUISITES: BI 107; CH 101 or consent of department. Two hours lecture, three hours laboratory each week.
HELP	ARTD-Arts   BSSD-Behav. & Social Sci.   HUMD-Humanities    NSLD-Nat. Sci. with Lab   NSND-Nat. Sci. NO lab   [M] - Global & Cultural Prospective CE -- Credit by Exam available     (...only)  -- offered only on indicated campus(es)     G - Germantown    R - Rockville    TP - Takoma Park/Silver Spring
Course	Course Title	Semester Hours	Subject
BT 117L	CELL CULTURE & CELL FUNCTION	No Credit	BT-Biotechnology
Course Description:    Laboratory component of BT 117.
BT 200	PROTEIN BIOTECHNOLOGY	4 semester hours	BT-Biotechnology
Course Description:    This course provides an introduction to protein structure and function. Topics include primary, secondary, tertiary, and quarternary structure. Peptide and protein synthesis and translation systems for protein production are considered along with preservation of structure/function. Functional assays for proteins including basic principles of enzymology, enzyme kinetics, and binding assays are discussed. Strategies and methods of protein purification are considered with emphasis on chromatographic and electrophoretic techniques. Principles of proteomics including peptide mapping and sequencing. Diagnostic, therapeutic, and industrial applications of protein products are discussed. (G only) PREREQUISITES: BI 107; CH 120 or consent of department. Three hours lecture, three hours laboratory each week.
BT 200L	PROTEIN BIOTECHNOLOGY	No Credit	BT-Biotechnology
Course Description:    Laboratory component of BT 200.
BT 204	BASIC IMMUNOLOGY AND METHODS	4 semester hours	BT-Biotechnology
Course Description:    A brief survey of the components of the immune system and how they interact. B and T cell development, activation and culture, the role of cytokines, their production and purification, signal transduction processes in B-cell activation, the role of MHC complexes, immunoglobulin synthesis and origins of diversity, antigen-antibody interactions, practical aspects of raising and purifying polyclonal and monoclonal antibodies, handling and labeling of antibodies, applications of antibodies including Western blotting, ELISA, and immunohistochemistry. (G only) (CE) PREREQUISITES: BT 117; BT 200 or consent of department. Three hours lecture, three hours laboratory each week.
HELP	ARTD-Arts   BSSD-Behav. & Social Sci.   HUMD-Humanities    NSLD-Nat. Sci. with Lab   NSND-Nat. Sci. NO lab   [M] - Global & Cultural Prospective CE -- Credit by Exam available     (...only)  -- offered only on indicated campus(es)     G - Germantown    R - Rockville    TP - Takoma Park/Silver Spring
Course	Course Title	Semester Hours	Subject
BT 204L	BASIC IMMUNOLOGY AND METHODS	No Credit	BT-Biotechnology
Course Description:    Laboratory component of BT 204.
BT 213	NUCLEIC ACID METHODS	4 semester hours	BT-Biotechnology
Course Description:    An introduction to current methods and theory of basic molecular techniques used in the study of nucleic acids. Lecture topics include structure of DNA and RNA, DNA isolation and sequencing, an introduction to genomics and bioinformatics, probe design and hybridization, DNA replication, PCR, microarrays, RNA isolation, regulation of prokaryotic and eukaryotic gene expression, enzymes used in molecular biology, principles of cloning including the use of vectors for sequencing and expression. (G only) PREREQUISITES: BI 203 and CH 120; BT 200 or consent of department. Three hours lecture, three hours laboratory each week.
BT 213L	NUCLEIC ACID METHODS	No Credit	BT-Biotechnology
Course Description:    Laboratory component of BT 213.
BT 235	PRINCIPLES OF BIOMANUFACTURING	4 semester hours	BT-Biotechnology
Course Description:    An introduction to the process of producing a biological product using a cell line. The course will be organized as a production campaign in a simulated cGMP environment. Students will complete a batch record as they produce a biological product. Emphasis will be on upstream and downstream processes. Hands-on laboratory work will involve the preparation and qualification of growth media and chromatography buffers, the use of bioreactors and FPLC protein purification systems. The role of QA/QC will be discussed. (G only) PREREQUISITE: BT 117; BT 200 or consent of department. Three hours lecture, three hours laboratory each week.
HELP	ARTD-Arts   BSSD-Behav. & Social Sci.   HUMD-Humanities    NSLD-Nat. Sci. with Lab   NSND-Nat. Sci. NO lab   [M] - Global & Cultural Prospective CE -- Credit by Exam available     (...only)  -- offered only on indicated campus(es)     G - Germantown    R - Rockville    TP - Takoma Park/Silver Spring
Course	Course Title	Semester Hours	Subject
BT 235L	PRINCIPLES OF BIOMANUFACTURING	No Credit	BT-Biotechnology
Course Description:    Laboratory component of BT 235.

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